CCR5 (GP-non-phospho) Chemokine Receptor 5 Antibody, Guinea Pig

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Order number: 7TM0062N-GP
Content: 100 µl
Host: Rabbit

The non-phospho-CCR5 receptor antibody is directed against the distal end of the... more

The non-phospho-CCR5 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human CCR5. It can be used to detect total CCR5 receptors in Western blots independent of phosphorylation. The CCR5 antibody can also be used to isolate and enrich CCR5 receptors from tissue lysates.

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Alternative Names CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif)... more

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Alternative Names	CCR5, CKR5, Chemokine Receptor 5, Chemokine (C-C motif) Receptor 5, MIP-1 alpha Receptor
IUPHAR Target ID	62
UniProt ID	P51681
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CCR5 around the phosphorylation site of Thr340.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5. Upper... more

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Figure 1. Agonist-induced Threonine340 phosphorylation of the Chemokine Receptor 5. Upper panel, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to 100 nM specific Chemokine receptor 5 agonist CCL5 or 0.1 μM PMA (Phorbol 12-Myristate 13-Acetate) for 30 minutes. Cells were lysed and immunoblotted with the anti-pT340-CCR5 antibody (7TM0062B) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.

Figure 2. Analysis of dose-dependent Chemokine Receptor 5 phosphorylation using three phosphosite-specific antibodies. Upper three panels, HEK293 cells stably expressing the Chemokine Receptor 5 (CCR5) were either not exposed or exposed to increasing concentrations of specific Chemokine receptor 5 agonist CCL5 ranging from 3 nM to 100 nM for 30 minutes. Cells were lysed and immunoblotted with the anti-pS336/pS337-CCR5 antibody (7TM0062A) or pT340-CCR5 antibody (7TM0062B) or pS342-CCR5 antibody (7TM0062C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-CCR5 antibody to confirm equal loading of the gel.

Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK,... more

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Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK, Grzesiek S. A key GPCR phosphorylation motif discovered in
arrestin2⋅CCR5 phosphopeptide complexes. Mol Cell. 2023 Jun 15;83(12):2108-2121.e7. doi: 10.1016/j.molcel.2023.05.002. Epub 2023 May 26. PMID: 37244255.

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