CXCR4 (GP-non-phospho), CXC Chemokine Receptor 4 Antibody, Guinea Pig

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Order number: 7TM0071N-GP
Content: 100 µl
Host: Rabbit

The non-phospho-CXCR4 receptor antibody is directed against the distal end of the... more

The non-phospho-CXCR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human CXCR4. It can be used to detect CXCR4 receptors in Western blots in a phosphorylation-sensitive manner. After agonist activation CXCR4 is phosphorylated at S346/S347. Because the CXCR4 antibody detects the same epitope, it no longer binds to the receptor. It detects selectively CXCR4 receptors that have been hot activated and not phosphorylated. Total CXCR4 can be detected in Western blots and immunohistochemistry only after phosphatase treatment. It can also be used to isolate and enrich CXCR4 receptors from brain lysates. It also detects CXCR4 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names CXCR4, CXC Chemokin Receptor 4 IUPHAR Target ID... more

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Alternative Names	CXCR4, CXC Chemokin Receptor 4
IUPHAR Target ID	71
UniProt ID	P61073 (human) P70658 (mouse) O08565 (rat)
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	1:200
Immunohistochemisty (IHC)	1:100
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide with the sequence KGKRGGHSSVSTESESSSFHSS corresponding to residues 338-359 in human, mouse and rat CXCR4
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the CXC Chemokine Receptor 4 in transfected HEK293 cells . Native... more

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Figure 1. Validation of the CXC Chemokine Receptor 4 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the CXC Chemokine Receptor 4 (CXCR4) were lysed and immunoblotted with the phosphorylation-sensitive anti-CXCR4 antibody (7TM0071N-WB) at a dilution of 1:1000.

Stumm RK, Rummel J, Junker V, Culmsee C, Pfeiffer M, Krieglstein J, Höllt V, Schulz S. A dual... more

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Stumm RK, Rummel J, Junker V, Culmsee C, Pfeiffer M, Krieglstein J, Höllt V, Schulz S. A dual role for the SDF-1/CXCR4 chemokine receptor system in adult brain: isoform-selective regulation of SDF-1 expression modulates CXCR4-dependent neuronal plasticity and cerebral leukocyte recruitment after focal ischemia. J Neurosci. 2002 Jul 15;22(14):5865-78. PubMed PMID: 12122049; PubMed Central PMCID: PMC6757949.

Stumm RK, Zhou C, Ara T, Lazarini F, Dubois-Dalcq M, Nagasawa T, Höllt V, Schulz S. CXCR4 regulates interneuron migration in the developing neocortex. J Neurosci. 2003 Jun 15;23(12):5123-30. PubMed PMID: 12832536; PubMed Central PMCID: PMC6741192.

Fischer T, Nagel F, Jacobs S, Stumm R, Schulz S. Reassessment of CXCR4 chemokine receptor expression in human normal and neoplastic tissues using the novel rabbit monoclonal antibody UMB-2. PLoS One. 2008;3(12):e4069. doi: 10.1371/journal.pone.0004069. Epub 2008 Dec 31. PubMed PMID: 19116653; PubMed Central PMCID: PMC2605258.

Sánchez-Alcañiz JA, Haege S, Mueller W, Pla R, Mackay F, Schulz S, López-Bendito G, Stumm R, Marín O. Cxcr7 controls neuronal migration by regulating chemokine responsiveness. Neuron. 2011 Jan 13;69(1):77-90. doi: 10.1016/j.neuron.2010.12.006. PubMed PMID: 21220100.

Gourni E, Demmer O, Schottelius M, D'Alessandria C, Schulz S, Dijkgraaf I, Schumacher U, Schwaiger M, Kessler H, Wester HJ. PET of CXCR4 expression by a (68)Ga-labeled highly specific targeted contrast agent. J Nucl Med. 2011 Nov;52(11):1803-10. doi: 10.2967/jnumed.111.098798. PubMed PMID: 22045709.

Otte M, Kliewer A, Schütz D, Reimann C, Schulz S, Stumm R. CXCL14 is no direct modulator of CXCR4. FEBS Lett. 2014 Dec 20;588(24):4769-75. doi: 10.1016/j.febslet.2014.11.009. Epub 2014 Nov 18. PubMed PMID: 25451233.

Saaber F, Schütz D, Miess E, Abe P, Desikan S, Ashok Kumar P, Balk S, Huang K, Beaulieu JM, Schulz S, Stumm R. ACKR3 Regulation of Neuronal Migration Requires ACKR3 Phosphorylation, but Not β-Arrestin. Cell Rep. 2019 Feb 5;26(6):1473-1488.e9. doi: 10.1016/j.celrep.2019.01.049. PubMed PMID: 30726732.

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