pS330-CXCR4 (phospho-CXC Chemokine Receptor 4 Antibody)

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Preview: Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4

Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4

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Order number: 7TM0071C
Content: 100 µl
Host: Rabbit

Serine330(S330) is a major phosphorylation site of the CXCR4 receptor. The pS330-CXCR4 antibody... more

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Serine330(S330) is a major phosphorylation site of the CXCR4 receptor. The pS330-CXCR4 antibody detects phosphorylation in response to agonists and after PKC activation. S330 phosphorylation is a key regulator of CXCR4 desensitization, β-arrestin recruitment and internalization.

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Alternative Names CXCR4, CXC Chemokine Receptor 4 IUPHAR Target ID... more

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Alternative Names	CXCR4, CXC Chemokine Receptor 4
IUPHAR Target ID	71
UniProt ID	P61073 (human) P70658 (mouse)
Western Blot (WB)	1:1000
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human CXCR4 around the phosphorylation site of Ser330.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4. HEK293... more

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Figure 1. Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4. HEK293 cells transiently expressing CXCR4 were either not exposed or exposed to 100 nM CXCL12 for 5 minutes. Cells were lysed and immunoblotted with the anti-pS330 antibody (7TM0071C) at a dilution of 1:1000 (upper panel). The same lysates were probed with the non-phospho-CXCR4 antibody (7TM0071N) at a dilution of 1:1000 (lower panel).

Figure 2. Agonist-induced Serine330 phosphorylation of the CXC Chemokine Receptor 4. Flp-In Trex 293 cells induced to express the CXCR4 were either not exposed or exposed to 100 nM CXCL12 for 5 minutes. Cells were lysed and immunoblotted with the anti-pS330 antibody (7TM0071C) at a dilution of 1:1000 (upper panel). The same lysates were probed with the non-phospho-CXCR4 antibody (7TM0071N) at a dilution of 1:1000 (lower panel).

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