S1P1 (GP-IHC-grade), Sphingosine 1-Phosphate Receptor 1 Antibody, Guinea Pig

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Preview: Immunohistochemical identification of S1P1 Receptor in liver

Preview: Immunohistochemical identification of S1P1 Receptor in spleen

Preview: Validation of the S1P1 Receptor in transfected HEK293 cells

Immunohistochemical identification of S1P1 Receptor in liver

Immunohistochemical identification of S1P1 Receptor in spleen

Validation of the S1P1 Receptor in transfected HEK293 cells

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Order number: 7TM0275N-ICGP
Content: 100 µl
Host: Guinea Pig

The non-phospho-S1P1 receptor antibody is directed against the distal end of the... more

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The non-phospho-S1P1 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human S1P1. It also detects S1P1 in cultured cells and tissue sections by immunohistochemistry. It can be used to detect total S1P1 receptors in Western blots independent of phosphorylation. The S1P1 antibody can also be used to isolate and enrich S1P1 receptors from tissue lysates.

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Alternative Names CD363, Edg1, endothelial differentiation G... more

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Alternative Names	CD363, Edg1, endothelial differentiation G protein-coupled receptor 1 Receptor
IUPHAR Target ID	275
Uniprot ID	P21453
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human S1P1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of S1P1 Receptor in liver. Sections were... more

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Figure 1. Immunohistochemical identification of S1P1 Receptor in liver. Sections were dewaxed, microwaved in citric acid, and incubated with anti-S1P1 (non-phospho-Sphingosine 1-Phosphate Receptor 1) antibody (7TM0275N-ICGP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of S1P1 Receptor in spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-S1P1 (non-phospho-Sphingosine 1-Phosphate Receptor 1) antibody (7TM0275N-ICGP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Validation of the S1P1 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the S1P1 Receptor (S1P1) were lysed and immunoblotted with the phosphorylation-independent anti-S1P1 antibody (7TM0275N-ICGP) at a dilution of 1:100.