pT377-GPR88 (phospho-GPR88 Antibody)

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Agonist-induced Threonine377 phosphorylation of GPR88-Receptor

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Order number: 7TM0123D
Content: 100 µl
Host: Rabbit

Threonine377 (T377) is a major phosphorylation site of the human GPR88 receptor.... more

Threonine377 (T377) is a major phosphorylation site of the human GPR88 receptor. The pT377-GPR88 antibody detects phosphorylation in response to high-efficacy agonists. T377 phosphorylation is a key regulator of human GPR88 desensitization, β-arrestin recruitment and internalization.

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Alternative Names STRG, striatum-specific G-protein coupled receptor... more

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Alternative Names	STRG, striatum-specific G-protein coupled receptor
IUPHAR Target ID	123
UniProt ID	Q9GZN0
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human GPR88 around the phosphorylation site Thr377.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine377 phosphorylation of GPR88-Receptor. Upper panel,... more

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Figure 1. Agonist-induced Threonine377 phosphorylation of GPR88-Receptor. Upper panel, HEK293 cells stably expressing the GPR88 receptor (GPR88) and transiently GRK2 (Ratio GPR88 receptor:GRK2 = 10:1) were preincubated with serum-free media. After 2 hours cells were either not exposed or exposed to 100 nM of 2-PCCA for 30 minutes. Cells were lysed and immunoblotted with the anti-pT377-GPR88 antibody (7TM0123D) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-GPR88 antibody (7TM0123N) to confirm equal loading of the gel

Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA,... more

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Divorty N, Jenkins L, Ganguly A, Butcher AJ, Hudson BD, Schulz S, Tobin AB, Nicklin SA, Milligan G. Agonist-induced phosphorylation of orthologues of the orphan receptor GPR35 functions as an activation sensor. J Biol Chem. 2022 Mar;298(3):101655. doi: 10.1016/j.jbc.2022.101655. Epub 2022 Jan 29. PMID: 35101446; PMCID: PMC8892012.

Ganguly A, Quon T, Jenkins L, Joseph B, Al-Awar R, Chevigne A, Tobin AB, Uehling DE, Hoffmann C, Drube J, Milligan G. G protein-receptor kinases 5/6 are the key regulators of G protein-coupled receptor 35-arrestin interactions. J Biol Chem. 2023 Oct;299(10):105218. doi: 10.1016/j.jbc.2023.105218. Epub 2023 Sep 1. PMID: 37660910; PMCID: PMC10520886.

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