NPSR (non-phospho), Neuropeptide S Receptor Antibody

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Preview: Validation of the Neuropeptide S Receptor in transfected HEK293 cells

Preview: NPSR (non-phospho), Neuropeptide S Receptor Antibody

Validation of the Neuropeptide S Receptor in transfected HEK293 cells

NPSR (non-phospho), Neuropeptide S Receptor Antibody

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Order number: 7TM0302N
Content: 100 µl
Host: Rabbit

The NPSR antibody is directed against the distal end of the carboxyl-terminal tail of human... more

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The NPSR antibody is directed against the distal end of the carboxyl-terminal tail of human Neuropeptide S Receptor. It can be used to detect total NPS receptors in Western blots independent of phosphorylation. The NPSR antibody can also be used to isolate and enrich NPS receptors from cell and tissue lysates.

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Alternative Names VRR1, Vasopressin Receptor-related Receptor IUPHAR... more

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Alternative Names	VRR1, Vasopressin Receptor-related Receptor
IUPHAR Target ID	302
UniProt ID	Q6W5P4
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents part of carboxyl-terminal tail of human NPSR.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Neuropeptide S Receptor in transfected HEK293 cells. Native... more

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Figure 1. Validation of the Neuropeptide S Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Neuropeptide S Receptor (NPSR) were lysed and immunoblotted with the anti-NPSR antibody (7TM0302N) at a dilution of 1:1000. Note, NPSR forms stable multimers.

Figure 2. Immunohistochemical identification of NPSR in Duodenum. Sections were dewaxed, microwaved in citric acid, and incubated with anti-NPSR (NPS Receptor) antibody (7TM0302N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.