pT315/pT316-M4 (phospho-M4 Muscarinic Acetylcholine Receptor Antibody)

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Agonist-induced Threonine315/Threonine316 phosphorylation of the M4 Muscarinic Acetylcholine Receptor

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Order number: 7TM0016C
Content: 100 µl
Host: Rabbit

Threonine315/Threonine316 is a major phosphorylation site of the M4 Muscarinic Acetylcholine... more

Threonine315/Threonine316 is a major phosphorylation site of the M4 Muscarinic Acetylcholine Receptor. The pT315/pT316-M4 antibody detects phosphorylation in response to high-efficacy agonists but not after PKC activation. T315/T316 phosphorylation is a key regulator of M4 desensitization, β-arrestin recruitment and internalization.

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Alternative Names HM3, Chrm 4, Cholinergic Receptor muscarinic 4... more

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Alternative Names	HM3, Chrm 4, Cholinergic Receptor muscarinic 4
IUPHAR Target ID	16
UniProt ID	P08173
Western Blot (WB)	1:1000
Immunocytochemistry (ICC)	-
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human M4 around the phosphorylation site of Thr315/Thr316.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine315/Threonine316 phosphorylation of the M4 Muscarinic... more

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Figure 1. Agonist-induced Threonine315/Threonine316 phosphorylation of the M4 Muscarinic Acetylcholine Receptor. Upper panel, HEK293 cells stably expressing the M4 Muscarinic Acetylcholine Receptor (M4) and high level of GRK2 were either not exposed or exposed to 100 µM Carbachol for 30 minutes. Cells were lysed and immunoblotted according to 7TM Western Blot Protocol with the anti-pT315/pT316-M4 antibody (7TM0016C) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-M4 antibody (7TM0016N) to confirm equal loading of the gel.

Be the first to decipher the M4 phosphorylation barcode and let us know. more

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Be the first to decipher the M4 phosphorylation barcode and let us know.

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