HCA2 (GP-IHC-grade), Hydroxycarboxylic Acid Receptor 2 Antibody, Guinea Pig

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Preview: Immunohistochemical identification of the Hydroxycarboxylic Acid Receptor 2 in spleen

Preview: Validation of the Hydroxycarboxylic Acid Receptor 2 in transfected HEK293 cells

Immunohistochemical identification of the Hydroxycarboxylic Acid Receptor 2 in spleen

Validation of the Hydroxycarboxylic Acid Receptor 2 in transfected HEK293 cells

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Order number: 7TM0312N-ICGP
Content: 100 µl
Host: Guinea Pig

The non-phospho HCA2 receptor antibody is directed against the distal end of the... more

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The non-phospho HCA2 receptor antibody is directed against the distal end of the carboxyl-terminal tail human HCA2 receptor. It can be used to detect total HCA2 receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich HCA2 receptors from tissue lysates. It also detects HCA2 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names PUMAG, Nicotinic acid receptor, G-protein coupled... more

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Alternative Names	PUMAG, Nicotinic acid receptor, G-protein coupled receptor 109A, Niacin receptor 1
IUPHAR Target ID	312
UniProt ID	Q8TDS4
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human HCA2.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification... more

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Figure 1. Immunohistochemical identification of the Hydroxycarboxylic Acid Receptor 2 in spleen. Sections were dewaxed, microwaved in citric acid, and incubated with anti-HCA2 (Hydroxycarboxylic Acid Receptor 2) antibody (7TM0312N-ICGP) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Validation of the Hydroxycarboxylic Acid Receptor 2 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Hydroxycarboxylic Acid Receptor 2 (HCA2) were lysed and immunoblotted with the anti-HCA2 antibody (7TM0312N-ICGP) at a dilution of 1:1000.