HCA1 (non-phospho) Hydroxycarboxylic Acid Receptor 1 Antibody

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Preview: Validation of the Hydroxycarboxylic Acid Receptor 1 in transfected HEK293 cells

Preview: Immunohistochemical identification of the Hydroxycarboxylic Acid Receptor 1 in pancreas

Validation of the Hydroxycarboxylic Acid Receptor 1 in transfected HEK293 cells

Immunohistochemical identification of the Hydroxycarboxylic Acid Receptor 1 in pancreas

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Order number: 7TM0311N
Content: 100 µl
Host: Rabbit

The non-phospho HCA1 receptor antibody is directed against the distal end of the... more

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The non-phospho HCA1 receptor antibody is directed against the distal end of the carboxyl-terminal tail human HCA1 receptor. It can be used to detect total HCA1 receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich HCA1 receptors from tissue lysates.

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Alternative Names FKSG80, LACR1, lactate receptor 1, T-cell activation G... more

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Alternative Names	FKSG80, LACR1, lactate receptor 1, T-cell activation G protein-coupled receptor
IUPHAR Target ID	311
UniProt ID	Q9BXC0
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human HCA1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Hydroxycarboxylic Acid... more

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Figure 1. Validation of the Hydroxycarboxylic Acid Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Hydroxycarboxylic Acid Receptor 1 (HCA1) were lysed and immunoblotted with the anti-HCA2 antibody (7TM0311N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of the Hydroxycarboxylic Acid Receptor 1 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-HCA1 (Hydroxycarboxylic Acid Receptor 1) antibody (7TM0311N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.