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- Order number: 7TM0252N
- Content: 100 µl
- Host: Rabbit
The non-phospho SCTR receptor antibody is directed against the distal end of the carboxyl-terminal tail human SCTR receptor. It can be used to detect total SCTR receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich SCTR receptors from tissue lysates. It also detects SCTR in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | SCT-R |
| IUPHAR Target ID | 252 |
| UniProt ID | P47872 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to c-terminal end of human SCTR. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the Secretin Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Secretin Receptor (SCTR) were lysed and immunoblotted with the phosphorylation-independent anti-SCTR antibody (7TM0252N) at a dilution of 1:100.
Figure 2. Immunohistochemical identification of Secretin Receptor in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SCTR (non-phospho-Secretin Receptor) antibody (7TM0252N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 3. Immunohistochemical identification of Secretin Receptor in rat cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SCTR (non-phospho-Secretin Receptor) antibody (7TM0252N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 4. Immunohistochemical identification of Secretin Receptor in human cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SCTR (non-phospho-Secretin Receptor) antibody (7TM0252N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.