C3a (non-phospho), Complement C3a Receptor Antibody

Figure 1. Validation of the Complement C3a Receptor 1 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the complement C3a receptor  (C3a) were lysed and immunoblotted with the phosphorylation-independent anti-C3a antibody (7TM0031N) at a dilution of 1:1000.

Figure 2. Immunohistochemical varification of complement C3a Receptor antibody in human spleen. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-C3a (non-phospho-complement C3a Receptor) antibody (7TM0031N). Sections were then incubated with anti-C3a (non-phospho-C3a Receptor) antibody (7TM0031N) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation C3a receptors were uniformly detected at the plasma membrane of nearly all cells.