5-HT4 (IHC-grade), 5-HT Receptor 4 Antibody

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Preview: Immunohistochemical identification of 5-Hydroxytryptamine Receptor 4 in kidney

Preview: Immunohistochemical identification of 5-Hydroxytryptamine Receptor 4 in pancreas

Immunohistochemical identification of 5-Hydroxytryptamine Receptor 4 in kidney

Immunohistochemical identification of 5-Hydroxytryptamine Receptor 4 in pancreas

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Order number: 7TM0009N-IC
Content: 100 µl
Host: Rabbit

The 5-HT4 receptor antibody is directed against the distal part of the carboxyl-terminal tail of... more

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The 5-HT4 receptor antibody is directed against the distal part of the carboxyl-terminal tail of human 5-Hydroxytryptamine Receptor 4 (5-HT4). It can be used to detect total 5-HT4 receptors in Western blots independent of phosphorylation. The non-phospho-5-HT4 antibody can also be used to isolate and enrich 5-HT4 receptors from cell and tissue lysates. It also detects 5-HT4 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names 5-HT4, 5-Hydroxytryptamine Receptor 4, Serotonin Receptor... more

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Alternative Names	5-HT4, 5-Hydroxytryptamine Receptor 4, Serotonin Receptor 4
IUPHAR Target ID	9
UniProt ID	Q13639
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human 5-HT4.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical... more

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Figure 1. Immunohistochemical identification of 5-Hydroxytryptamine Receptor 4 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-5-HT4 (5-Hydroxytryptamine Receptor 4) antibody (7TM0009N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of 5-Hydroxytryptamine Receptor 4 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-5-HT4 (5-Hydroxytryptamine Receptor 4) antibody (7TM0009N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

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