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- Order number: 7TM0084N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR4. It can be used to detect total GPR4 receptors in Western blots independent of phosphorylation. The GPR4 antibody can also be used to isolate and enrich GPR4 receptors from tissue lysates. It also detects GPR4 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | Gpcr21, GPCR3, ACCA orphan receptor |
| IUPHAR Target ID | 83 |
| Uniprot ID | P46089 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR3. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the GPR3 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR3 Receptor (GPR3) were lysed and immunoblotted with the phosphorylation-independent anti-GPR3 antibody (7TM0083N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of GPR3 Receptor in Cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR3 (GPR3 Receptor) antibody (7TM0083N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-guinea pig IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.