EP4 (GP-non-phospho), EP4 Prostanoid Receptor Antibody, Guinea Pig

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Preview: Indentification of EP4 Receptor in mouse tissues

Preview: Validation of the EP4 Receptor in transfected HEK293 cells

Indentification of EP4 Receptor in mouse tissues

Validation of the EP4 Receptor in transfected HEK293 cells

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Order number: 7TM0343N-GP
Content: 100 µl
Host: Guinea Pig

The non-phospho-EP4 receptor antibody is directed against the distal end of the... more

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The non-phospho-EP4 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human EP4. It can be used to detect total EP4 receptors in Western blots independent of phosphorylation.

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Alternative Names PGE Receptor EP4 subtype, Prostanoid EP4 Receptor... more

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Alternative Names	PGE Receptor EP4 subtype, Prostanoid EP4 Receptor
IUPHAR Target ID	343
Uniprot ID	P35408
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Guinea Pig / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human EP4.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Indentification of EP4 Receptor in mouse tissues. Native mouse tissues were... more

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Figure 1. Indentification of EP4 Receptor in mouse tissues. Native mouse tissues were lysed and GPCR receptors were enriched by using WGA-beads (see 7TM Western Blot Protocol). Extracted proteins were immunoblotted with the phosphorylation-independent anti-EP4 antibody (7TM0343N-GP) at a dilution of 1:1000.

Figure 2. Validation of the EP4 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the EP4 Receptor (EP4) were lysed and immunoblotted with the phosphorylation-independent anti-EP4 antibody (7TM0343N-GP) at a dilution of 1:1000.

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