β1 (GP-IHC-grade), β1-Adrenoceptor Antibody, Guinea Pig

Figure 1. Immunohistochemical identification of ß1-Adrenoceptor in Heart. Sections were dewaxed, microwaved in citric acid, and incubated with anti-ß1 (ß1-Adrenoceptor) antibody (7TM0028N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-guinea pig IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the β1-Adrenoceptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the β1-Adrenoceptor (β1) were lysed and immunoblotted with the phosphorylation-independent anti-β1 antibody (7TM0028N-ICGP) at a dilution of 1:1000.

Figure 3. Immunohistochemical varification of  ß1-Adrenoceptor antibody in heart. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-ß1 (non-phospho-ß1-Adrenoceptor)) antibody (7TM0028N-ICGP). Sections were then incubated with anti-ß1 (non-phospho-ß1-Adrenoceptor) antibody (7TM0028N-ICGP) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation ß1 receptors were uniformly detected at the plasma membrane of cells.

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